Anole Annals

Late Breaking: one last Evolution 2017 post!  Last weekend during the Evolution meeting, I had a chance to chat with Iris Holmes (Ph.D. student, University of Michigan) about the poster she presented. Initially not on our watch list because of the lack of “anole” in the description, my eye caught the dewlapping lizard perched at the top of her poster from across the room.

Iris presented her work on gut microbiomes of two groups of lizards: anoles and ameivas. She wanted to know if different taxa have different gut microbiomes and to what extent diet influences bacterial composition of gut microbiomes. Her collaborator (Ivan Monagan) collected scat samples from 22 Anolis dollfusianus and 9 Ameiva from an agricultural area in the Soconosco region of Chiapas, Mexico. Together, they then sequenced both the gut bacteria and the digesting prey with two 16S primers. Iris chose to target the prey as well because she wanted to know if they were eating different things and how different stages of digestion influence gut bacteria communities.

Iris found that there were no clear differences between the gut microbiomes of anoles and ameivas. Both species had gut microbiomes dominated by three main phyla: Proteobacteria, Firmicutes, and Bacteroidetes. Little is currently known about how these bacteria relate to digestion and health in reptiles, but Iris commented that we can make some guesses based on studies in other taxa. Proteobacteria are a disease indicator in mammals, but appear to be normal in reptiles and birds. Firmicutes and Bacteroidetes are both important for digestion of carbohydrates and fats (respectively) in mammals. Iris found that there was a loose correlation between the amount of prey consumed and the abundance of Bacteroidetes, suggesting these bacteria also play a role in digestion in lizards. She also found that there was an apparent tradeoff between the Proteobacteria and the two other groups – sequence abundance of proteobacteria was negatively correlated with abundance of Bacteroidetes and Firmicutes. Overall, this is an interesting first step in understanding the gut microbiomes of reptiles and how they differ (or don’t) between groups.

Major Anole Annals contributor Martha Muñoz gave a brilliant talk at the Evolution meeting  as an awardee of a well-deserved ‘Young Investigator’ award from the American Society of Naturalists. In her talk, Muñoz discussed how two classic papers by Janzen (1967) and Huey et al. (2003) influenced the way she thinks about the interplay between behavior, physiology, and evolution. Not surprisingly, Anolis lizards played a leading role in her exposition.

Martha Muñoz introduces the Cybotoid Anoles.

Martha’s talk, entitled “Janzen’s hypothesis meets the Bogert effect: a synthesis nearly 100 years in the making”, started by describing Janzen’s hypothesis. In short, Janzen (1967) predicted that physiological differences among populations across altitudinal bands would be stronger in tropical mountains than in temperate ones. The main argument was that populations can more easily adapt to a given temperature range in tropical environments because these ranges are stable throughout the year, whereas the temperatures of different altitudinal bands overlap more in temperate areas due to seasonal variation.

Martha explains how day and night temperatures in the tropics mirror seasonal patterns in temperate and tropical climates.

Expanding on Janzen’s idea, Muñoz hypothesized that diurnal and nocturnal temperature variation in a single tropical mountain could also generate differences in physiological divergence among lowland and highland populations. The idea was that daytime temperatures were variable with overlap across elevation (similar to the seasonal picture in temperate areas) and nighttime temperatures were more constant and differed between elevations (similar to the seasonal picture in tropical areas).

To test this, Martha sampled populations in the Dominican Republic at sites ranging in elevation from sea level to 2400m. She then analyzed heat and cold tolerance of several species of anoles from the Anolis cybotes group. Results on cold tolerance (CT min) seem to agree with Janzen’s hypothesis: cold tolerance strongly covaries with altitude at night, with higher elevation populations having lower critical thermal minimums. Interestingly, however, heat tolerance (measured as CT max) was not at all associated with elevation.

Why did Janzen’s hypothesis fail to explain the evolution of heat tolerance across the altitudinal range? This question led to a key point of Muñoz’s talk: Janzen’s hypothesis might fail to predict evolution of CT max because it is agnostic about behavior. In the case of ‘cybotoid’ anoles, lizards from different altitudes could actively adjust their habitat use to achieve optimal temperatures. As a consequence, thermoregulatory behavior could forestall evolution of physiology in heat tolerance. By studying habitat use across different elevations, Muñoz showed that, although anoles behave as thermo-conformers at low elevations, they clearly thermoregulate at high elevations. In other words, anoles were at similar temperatures to the average available substrates in lowlands but their body temperatures were significantly higher than perches at higher elevation.

Martha explains how the thermoregulation can lead to slower evolution in a trait (the Bogert effect)

This was at least partially explained by habitat use differences: anoles at high elevations perched most frequently on boulders, which are on average about 5º C warmer than trees –the most used substrate in low altitudes. In fact, 90% of the trees Martha sampled at these high elevation sites were lower in temperature than the preferred temperature of the lizards! These data indicate that anoles from the A. cybotes group have buffered natural selection in physiology by means of behavioral adjustments –a phenomenon known as the Bogert effect (also called behavioral inertia; Bogert 1949).

Finally, the talk had a third part. And yes, it got even more interesting! Due to the observed habitat use differences in high latitudes, Muñoz and her collaborators predicted that although behavior could buffer physiological evolution on heat tolerance, it could spur evolutionary change in ecologically-relevant morphological traits (the behavioral drive hypothesis). Specifically, they predicted that increased use of boulders (for thermoregulation) at high elevations should drive morphological shifts in traits related to boulder use: head and limb morphology. They found evidence for these hypothesized morphological differences: high elevation lizards had higher head heights and longer hindlimb,  in agreement with functional predictions. Finally, a captive breeding experiment confirmed that these differences were the consequence of genetic changes and not simply due to developmental plasticity.

Martha’s research is a great example of how, as Huey said, studying behavior can be crucial to improve our understanding of evolutionary processes. We are looking forward to hear about future research from the Muñoz lab, which is about to open at Virginia Tech!

References:
Janzen, D.H. 1967. Why mountain passes are higher in the tropics. American Naturalist 101:233–249

Huey, R.B., Hertz, P.E., Sinervo, B. 2003. Behavioral drive versus behavioral inertia in evolution: a null model approach. American Naturalist 161: 357–366.

Muñoz, M.M. et al. 2014b. Evolutionary stasis and lability in thermal physiology in a group of tropical lizards. Proc. R. Soc. B 281: 20132433.

Muñoz, M.M., Losos, J.B. Thermoregulation simultaneously promotes and forestalls evolution in a tropical lizard. (Accepted pending minor revision). American Naturalist.

In his Masters thesis conducted in Simon Lailvaux’s lab at the University of New Orleans and presented this week at Evolution 2017, David Weber used a multiyear data set of Anolis carolinensis lizards’ locations and morphology as well as a DNA-based pedigree to investigate the effects of body size and relatedness on the spatial distribution of these lizards. Specifically, he set out to test three hypotheses: first, are males’ home ranges larger than females’ home ranges? Second, are bigger males more likely to be surrounded by smaller males that are related to them? And third, is there any evidence for the inheritance of home ranges from parent to offspring?

Anolis carolinensis dewlapping. Photo by Cowenby available on Wikipedia.

Lizard locations were sampled in an urban New Orleans park twice a year, in the fall and in the spring, from 2010 to 2015. The dataset included over 800 individuals, and what struck me most about these data was that, of these 800+ individuals, fewer than 100 were observed often enough to estimate home range volumes–death and dispersal can rule these lizards’ lives! Male and female home range volumes did not differ significantly (and the trend was in the direction of females moving over larger areas, which concurs with data from Robert Gordon’s 1956 thesis on green anoles, but with little else, I think). Curiously, smaller neighbours of the biggest males were less related to them than were males found farther away, suggesting that male anoles don’t preferentially tolerate their kin over non-kin. And though philopatry  (aka site fidelity aka staying the same place) was rare overall, females were a bit more likely to co-occur with their male offspring than males were. In a result that conforms to traditional wisdom, Weber found that the biggest males in the site seemed to avoid each other, potentially spacing themselves as far apart as possible.

Following a kind shout-out to my and Jonathan Losos’ recent paper on Anolis territoriality or the lack thereof, Weber chose to interpret his results as making sense only outside of a territorial framework. Unsurprisingly, I concur with this decision entirely, and am excited to see where Weber goes with this idea in the publications resulting from this mammoth dataset!

The arrival of an outsider that overlaps in resource use and habitat with local species can lead to intense competition between the two. A result of this competition can be character displacement, where traits of the species (one or both) change in sympatric populations (where the co-occur), but not in allopatric populations. Claire Dufour (Post-Doctoral researcher at Harvard University) presented her work on character displacement for two anole species on the island of  Dominica: the native Anolis oculatus and the introduced Anolis cristatellus. Her objective was to integrate ecological, antagonistic and reproductive character displacement. Specifically, she tested whether competition  between these new island-mates leads to changes in habitat use, morphology, and display behavior.

Location of populations of the introduced A. cristatellus with the sampled area, Calibishie inset

Claire compared allopatric populations of the two species with sympatric populations in the northern area of the island in Calibishie, where Anolis cristatellus has been present for two years. She found that in sympatry, both morphological and behavioral shifts have occurred. In sympatry, Anolis oculatus perched higher and had shorter limbs. She also found differences in display behavior, which she tested with an anole robot programmed to dewlap and do push-ups. This experiment showed that in sympatry, Anolis cristatellus dewlapped less, but Anolis oculatus does not alter its display behavior. Future work will test for reproductive character displacement and contrast populations where Anolis cristatellus has been present for a longer time span.

The Sensory Drive hypothesis predicts that species will evolve communication signals that are effective in the particular light environment in which they occur. Anolis lizards are an excellent example: in dark habitats, they tend to have light-colored, highly reflective (and transmissive) dewlaps that are usually yellow or white in color, whereas in bright, open environs, dewlaps tend toward blue, black, orange or red. However, demonstrating that these dewlaps are actually effective at being visible in their particular habitats has proven surprisingly challenging.

Leo Fleishman has been a leader in this area and in a talk at the sensory ecology symposium at the evolution meetings, he presented new and exciting developments. First, in line with previous work, he showed that the spectral reflectance/transmittance of dewlaps is not particularly well-matched to that of the background. Rather, the same colored dewlaps appear to be maximally contrasting with the radiance of the background across all habitats:  basically all habitats have mostly green backgrounds, and red or orange stands out the best against the green background, no matter what the habitat.  So much for sensory drive, it would seem!

But more recent work saves the day: it turns out that habitats differ in the total intensity of light (number of photons coming down) they receive and that, furthermore, across species, dewlap intensity (total photons reflected and/or transmitted) is negatively related to habitat intensity (with one notable outlier, the enigmatic A. gundlachi). Under the relatively low light conditions of forest shade or partial shade, color discrimination becomes more difficult, and colors such as red and orange and other dark colors do not stand out well against the background, because they simply do not emit enough photons to efficiently drive color vision.  Yellow or white works better. Conversely, in intense light environments, there is enough light to easily see the darker colors, and these stand out well against the green background. Moreover, behavioral experiments confirm that in bright light conditions red stimuli are most visible against a green background, whereas in low light yellow stimuli are more visible.  Thus, even though most Anolis habitats have similar spectral properties, differences in total light intensity strongly influence what colors are most effective, and thus appear to have played a major role in the shaping the evolution of dewlap colors.

Leo Fleishman discusses color space in four dimensions, corresponding to the four cones in the anole eye. For each species, red dots are color of the dewlap and green dots are the color of the background, indicating that dewlaps stand out against their background.

Bright and early on the last day of the annual Evolution meeting, James Stroud (Florida International University) presented his work on character displacement in novel communities of introduced anoles in Miami. In this elegant use of a natural experiment, James looked at the novel co-existence of two anoles in their introduced range and wondered if character displacement was occurring as predicted when two ecologically similar species are found in sympatry. Specifically, James wanted to know if Anolis cristatellus and Anolis sagrei would shift their habitat use when in sympatry, resulting in correlated shifts in morphology. These species are both trunk-ground anoles of roughly the same body size. They are native to Cuba/Bahamas and Puerto Rico (respectively) and are diverged by ~50 million years.

James hypothesized that in their introduced range in Florida, these two species would diverge ecologically in sympatry but be more similar in allopatry. He found that in allopatry, both species attained similar relative abundances and perched at similar heights. However, in sympatry, both decline in relative abundance suggesting that these species are interacting strongly with one another. Even more interesting, in sympatry A. sagrei perches lower and spends more time on the ground than it does in allopatry, while A. cristatellus perches higher!

Next James hypothesized that these ecological shifts could lead to shifts in morphology. If A. sagrei is spending more time on the ground, perhaps longer limbs would be favored. Similarly, if A. cristatellus is spending more time higher up in the trees, perhaps there would be selection for stickier toepads. As predicted, A. sagrei had longer forelimbs and hindlimbs in sympatry. However, he did not find any difference in toepad morphology between sympatric and allopatric populations of A. cristatellus. Instead, he observed that A. cristatellus in sympatry with A. sagrei had significantly smaller heads.

James ended by wondering if alternative behavioral and social mechanisms may drive these observed shifts in head morphology. Either way, this case study provides an interesting insight into how a complex range of adaptive responses can result from a seemingly simple ecological interaction.

Cities and urban areas are expanding rapidly around the world, altering the environment and creating very different ecological and selective pressures for organisms that live in urban habitats. A few of the most striking differences between urban and natural habitats are higher temperatures and a huge increase in artificial substrates like the walls of buildings. These artificial substrates (e.g., metal, concrete) are not only significantly smoother than natural (i.e., trees) substrates, but also absorb, retain, and radiate heat differently. Consequently, organisms may alter their behavior to better deal with these and other challenges of city life. Since anoles cannot internally regulate their temperature, behavioral shifts may be driven by perch substrate properties, temperature, or some interaction of the two.

Kevin Aviles-Rodriguez (U. Mass. Boston) addressed this question in urban Anolis cristatellus in San Juan, Puerto Rico. He created experimental enclosures in which each wall was a different substrate: wood, plastic, painted cement, and metal. He placed individual lizards into the enclosures and observed which wall they were perched on throughout the day. He also recorded the temperature of each wall, to determine how perch temperature of each substrate type influenced perch choice. Aviles-Rodriguez conducted this experiment in both urban and forest populations, and predicted that urban lizards would use artificial substrates more readily than forest lizards.

Interestingly, he did not find that to be the case – lizards from both urban and forest habitats used bark much more than any other surface. However, when lizards did use artificial substrates, they tended to use metal and cement when these perches were cooler, suggesting that perch temperature is a factor in perch choice. Aviles-Rodriguez plans to test these hypotheses more thoroughly by conducting additional experiments across more urban replicates to see if the same pattern emerges. He also plans to experimentally control the temperatures of different perch substrates in his enclosures to see whether lizard choices are primarily driven by perch substrate or temperature.