Author: Anthony Gilbert Page 1 of 2

The Society for Integrative and Comparative Biology annual conference is happening next week, January 3-8 2022! With a return to in-person events, we are aiming to highlight the breadth of research talks and posters on anoles given by all the amazing junior researchers at the conference!

This post is a call-to-arms: we can’t cover all the presentations without your help! We are looking for interested folks attending the conference that can help the Annals by contributing blog posts featuring these researchers and their anoles. Folks that elected to do the virtual SICB+ option instead of attending in-person are also encouraged to get involved. If you’re interested, please contact Anthony for any additional details or information.

Below is a list of anole presentations happening at SICB 2022- please reach out if I missed any of any of these peaks both your scientific and blogging interests!

The end of 2019 is nigh, and soon people will be traveling home to spend time with loved ones, eating a superfluous amount of cookies and sweets, and of course, working on that poster or talk for the 2020 Society for Integrative and Comparative Biology (SICB) meeting in Austin, Texas. SICB is one of the largest national conferences for biologists, where an incredible diversity of research is presented each year. And of course, SICB meetings are home to several Anolis presentations, with approximately 45 or more talks and posters this year featuring anoles.

Every year at SICB, Anole Annals tries to cover as many of the anole talks and posters as we can, and this year is no exception! AA relies on conference attendees to blog about all of the awesome anole work being presented, with a strong focus on undergraduate and graduate research. If you’re a student or student researcher attending SICB, blogging for AA is a fantastic way of getting experience in communicating science with a broader audience. In addition, if you are presenting work on anoles at SICB this January and want to help us blog, we’ll return the favor and cover your work for the blog! Anybody can join the AA family- undergraduates, graduate students, postdocs, and faculty. If you’re interested in blogging for AA at SICB this year, please shoot me an email at anthony.gilbert09@gmail.com or leave a comment on this post and we can get you going. We can provide assistance, examples, and answer any questions you may have when it comes to blogging for AA. Thanks all!

Predicting the responses of species to current environmental and climate change is one of the largest duties of current biologists. Ectothermic species (including lizards) are particularly vulnerable because they lack the ability to metabolically generate heat and rely on environmental sources of temperature to maintain their body temperatures. For species that live in the tropics, this task is much harder because tropical environments experience less temperature variation both within and across seasons. Tropical lizards traverse these landscapes to try and maintain optimal and preferred body temperatures, but are all thermal environments equal in the constraints they impose on lizards?

To address this question, Lauren Neel, a student of Mike Angilletta’s at Arizona State University, collected an astounding amount of data from two species of anole: Anolis sagrei from their native range on Great Exuma in the Bahamas, and A. apletophallus in Panama. She collected environmental temperature data using biophysical models, thermal performance data by racing anoles at several different body temperatures and measuring their sprint speed, and preferred body temperatures by placing lizards in a thermal gradient. Despite both lizards living in tropical climates, she found distinct differences between the environments (and anoles!). Anolis sagrei thermoregulated more,  was active for longer periods of time than A. apletophallus, and exhibited warmer preferred temperatures. Neel and colleagues also found that A. sagrei is not likely to suffer a drop in performance capacity as environments warm over time, whereas A. apletophallus is likely to experience a significant reduction in their speed performance which might be a physiological precursor to population collapse and a rise in local extinction events. Great stuff coming from Lauren Neel; stay tuned for more!

We tend to think of exercise as a human activity–training our bodies in specific way to accomplish tasks, maintain strength and endurance, and live a healthier lifestyle. Wild animals exercise just as often if not more than humans, but the benefits to animal exercise have been somewhat contentious in biology. The responses to organismal exercise are very conserved with respect to evolutionary change and speciation. Those responses include an increase in performance capacity (such as speed or endurance), but often times trades-off with some other physiological trait, such as immunocompetence. With this trade-off looming, one outstanding question remains: does the exercise response enhance reproductive success and survival? Can we call this response “adaptive”?

To test this idea, Jerry Husak from the University of St. Thomas and his co-author, Simon Lailvaux at the University of New Orleans, measured the exercise response in 90 green anoles (Anolis carolinensis). They had 30 control lizards, 30 lizards trained with speed trials, and 30 lizards trained with endurance trials. They found that overall, exercise enhanced performance for all lizards relative to the controls, but training decreased the ability of a lizard to fight off an infection. They found that lizards that had been trained did not exhibit increases in movement rates, and also found that over time, trained lizards exhibited decreased survival relative to the controls! Is exercise actually bad for lizards?! Green anoles were twice as likely to survive when they were not trained, and differences in lizard body condition might be intimately linked with their probability of survival. Husak and Lailvaux are going to continue to test the idea that the exercise response confers some benefit to lizards outside of performance. Stay tuned!

Members of the same species share a common genome, the same set of genes and regulatory networks that build the proteins responsible for phenotypic diversity. However, that means that both of the sexes share a common genome, and that is an issue when males of a species are sensitive to their own environmental stimuli, and when females are required to invest a lot of their energy towards reproduction and rearing offspring. Species are often sexually dimorphic as well, where either the male or the female will be larger than their inter-sex counterpart, and this has to do with what ecological tasks each sex is responsible for. Do you have to defend a territory? You better be big! Do you want to create a lot of high-quality offspring? You better not invest that much energy into your own growth! It’s an interesting question in biology today: what happens genetically when a sexually-dimorphic species develops to adulthood?

To answer this question Albert Chung, a graduate student at Georgia Southern University, and his colleagues Robert Cox and Christian Cox designed an experiment to quantify changes in gene regulation in brown anoles throughout ontogeny and quantify how changes in gene regulation produce sexual dimorphism. They quantified gene expression at four different time points until adulthood across three different tissue types (brain, liver, and muscle) using RNA sequencing (RNAseq). Chung and colleagues found that sex-biased gene expression exhibits age specificity, with different age classes exhibiting different patterns of sexual dimorphism in gene expression. They also found that the number of sex-biased genes increases throughout development, important for a species to be able to develop both a larger sex and a smaller sex. In addition, sex-biased gene expression also varies among the different tissue types, with the liver exhibiting an increase in sex-biased genes throughout development, potentially to increase growth in male brown anoles compared to females! Chung spoke in the Raymond Huey Best Student Paper Award session for the Division of Ecology and Evolution and delivered a fantastic presentation. We look forward to learning more about the development of sexual dimorphism (especially in anoles!) from Albert and his co-authors.